How to Collect a Canine Urine Sample

By Elle Belmont


A canine urine sample is a valuable tool your vet uses to diagnose many diseases. For example, a urinary tract infection may cause symptoms such as painful urination, straining or dribbling in the house, while bloody or cloudy urine may indicate bladder, prostate or urethra disorders. And a dog that urinates excessively and drinks a lot of water may have diabetes insipidus, thyroid disorders or Cushing's disease. If your vet asks you to bring in a urine sample, you must typically collect the urine as a first-catch sample in the morning and bring it to your vet within 30 minutes, according to the Merck Veterinary Manual. If that doesn't fit in your schedule, you may refrigerate it for up to 24 hours, but ask your vet first. Urine is best examined as a fresh, newly collected sample and refrigeration may alter the results, leaving a need for another sample.

Step 1

Ask your vet if you must withhold water after midnight the evening before you collect the sample. Your vet may provide you with a sterile cup or bowl; if not, scrub a jar with hot water and a brush to remove any residue. Let the jar dry overnight. Wash a shallow pan or plant tray. Alternatively, place the jar or the shallow pan in the dishwasher and run the machine on "sanitize" wash and dry setting.

Step 2

Put on a pair of latex gloves and take your dog outside. Watch carefully for the moment when she is ready to squat. Quickly slide the shallow receptacle under the female dog from behind, and place it close to the ground. Catch a small amount of urine in the pan, then transfer it to a clean jar with a lid.

Step 3

Watch a male dog for the moment he lifts his leg, and place a jar close to the urine stream. Collect a small amount of urine and place the lid on the jar.

Step 4

Mark the jar with your dog's name, date and time the urine was collected. Alternatively, place the sealed jar in a plastic bag and write the data on the outside of the bag with a marker.

Step 5

Take your dog's urine sample to the vet as soon as possible, as recommended by your vet.
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